Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Anti-phospho-GADS (Thr262) Antibody, clone 6E11.2 is an antibody against phospho-GADS for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GRB2-related adapter protein 2 (UniProt O75791; also known as Adapter protein GRID, GRAP-2, GRB-2-like protein, Grb2-related adapter protein downstream of Shc, GRB2L, GRBLG, GRBX, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, P38, Protein GADS, SH3-SH2-SH3 adapter Mona) is encoded by the GRAP2 (also known as GADS, GRB2L, GRID) gene (Gene ID 9402) in human. GADS (Grb2-related adapter protein downstream of Shc), GRB2 and GRAP constitute the GRB2 family of adapter proteins characterized by a central SH2 domain (a.a. 58-149 of GADS) flanked between two SH3 domains (a.a. 1-56, 271-330 of GADS). Although the SH2 domains of all three members have similar binding specificities, the C-terminal SH3 domain of GADS possesses unique binding specificity that allows for its interaction with the adapter protein SLP-76 (SH2 domain-containing leukocyte protein of 76R01;kDa). The GADS-SLP-76 complex is recruited to tyrosine-phosphorylated LAT (linker for activation of T cells) via GADS SH2 domain, allowing the assembly of signaling complexes critical to T-cell activation. In addition, evidences indicate that GADS plays a critical role in the development of BCR-ABL-mediated lymphoid disease. The oncogenic Bcr-Abl fusion protein is shown to bind GADS in a phosphotyrosine-dependent manner and GADS is expressed in a subset of CML cell lines and B-ALL patient samples, where it associates with both BCR-ABL and SLP-76.

Immunogen

Epitope: pThr262.

KLH-conjugated linear peptide corresponding to a human GADS sequence containing phosphorylated Thr262.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GADS Thr262 phosphorylation induction in 10 µg lysate from Calyculin A- and TNFα-treated Jurkat cells.

Specificity

Clone 6E11.2 detects GADS phosphorylated on Thr262. The corresponding phosphorylation site in human GADS spliced isoform 2 is Thr149 (UniProt O75791-2).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed. 37.91 kDa (isoform 1) and 24.38 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.